American Society for Clinical Pathology (ASCP) Histotechnologist Practice Test

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What is a goal when preparing smears using the nickel method?

Ensuring a thin smear
Enhancing staining
Reducing air exposure
Increasing sample volume

The correct answer is: Reducing air exposure

The goal when preparing smears using the nickel method is primarily to ensure a thin smear. This method is specifically designed to create uniform thickness in the sample, which is critical for accurate evaluation under the microscope. A thin smear allows for better cellular detail to be seen during staining and imaging, which facilitates precise identification of cellular components and any pathological changes. While reducing air exposure can be beneficial in some contexts, the primary focus of the nickel method is the resultant quality of the smear itself. A thin smear improves the visibility of cells and their morphology, leading to more reliable diagnostic interpretations.